Discrepancy analysis, communication, and feedback for cytotechnologist quality improvement of nongynecologic cytopathology

The Clinical Laboratory Improvement Amendments of 1988 (CLIA '88) detail the requirements for the cytotechnologist (CT) who evaluates gynecologic cytopathology specimens. However, the role of the CT in nongynecologic cytopathology is not clearly defined. Furthermore, non gynecologic cytopathology cases are diverse and the screening, interpretative, and diagnostic issues may be quite different from the gynecologic cases. At our institution, the CT and pathologist review nongynecologic cytopathology cases. Since CLIA '88 does not require the CT to screen nongynecologic cytopathology cases, there are few guidelines for quality assessment or quality improvement for the CT regarding nongynecologic cytopathology cases. To provide better understanding of the expectations of the CT and the needs of the pathologist, we developed a system comparing the CT's interpretation to the pathologist's interpretation as a means for enhanced communication and feedback. Using our Laboratory Information System (LIS), we generate a daily report that lists all cases with discrepancy in diagnoses between the CT and pathologist. The general supervisor reviews this report for diagnostic discrepancy in each case. To determine the degree of discrepancy, numerical values are assigned to each primary interpretation. Minor discrepancies are defined as differences less than +/-2.0. Major discrepancies are defined as differences greater than or equal to +/-2.0. For the entire laboratory, the overall percentage of concordant cases was consistently above 80% for each of the 6 mo analysis. Regarding the monthly discrepancies, the proportion of minor discrepancies ranged from 11.09% to 15.44% and the proportion of major discrepancies ranged from 1.40% to 3.56%. The frequency distribution of discrepancies by degree approximates a normal (Gaussian) curve and serves as baseline information that may be used for comparison when there are changes in practice or personnel. The CTs attend slide review sessions conducted by the general supervisor for discussion of cases with major discrepancies. The discrepancy data from individual CTs are useful in counseling and recommending areas for improvement. As the CT and pathologist work cooperatively and in tandem, our system allows for a mechanism by which the expectations and needs of pathologist are communicated to the CT more effectively. We believe our process is a fundamental step in improving CT performance in Nongynecologic cytopathology and keeps the CT informed of complexities of nongynecologic cytopathology.     

Systemic and mucosal antibody responses specific to SARS-CoV-2 during mild versus severe COVID-19

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Autoimmune hepatitis in 828 Brazilian children and adolescents: clinical and laboratory findings,histological profile,treatments, and outcomes

ObjectiveThis large study with a long-term follow-up aimed to evaluate the clinical presentation, laboratory findings, histological profile, treatments, and outcomes of children and adolescents with autoimmune hepatitis.MethodsThe medical records of 828 children and adolescents with autoimmune hepatitis were reviewed. A questionnaire was used to collect anonymous data on clinical presentation, biochemical and histological findings, and treatments.ResultsOf all patients, 89.6% had autoimmune hepatitis-1 and 10.4% had autoimmune hepatitis-2. The female sex was predominant in both groups. The median age at symptom onset was 111.5 (6; 210) and 53.5 (8; 165) months in the patients with autoimmune hepatitis 1 and autoimmune hepatitis-2, respectively. Acute clinical onset was observed in 56.1% and 58.8% and insidious symptoms in 43.9% and 41.2% of the patients with autoimmune hepatitis-1 and autoimmune hepatitis-2, respectively. The risk of hepatic failure was 1.6-fold higher for autoimmune hepatitis-2. Fulminant hepatic failure occurred in 3.6% and 10.6% of the patients with autoimmune hepatitis-1 and autoimmune hepatitis-2, respectively; the risk was 3.1-fold higher for autoimmune hepatitis-2. The gamma globulin and immunoglobulin G levels were significantly higher in autoimmune hepatitis-1, while the immunoglobulin A and C3 levels were lower in autoimmune hepatitis-2. Cirrhosis was observed in 22.4% of the patients; biochemical remission was achieved in 76.2%. The actuarial survival rate was 93.0%. A total of 4.6% underwent liver transplantation, and 6.9% died (autoimmune hepatitis-1: 7.5%; autoimmune hepatitis-2: 2.4%).ConclusionsIn this large clinical series of Brazilian children and adolescents, autoimmune hepatitis-1 was more frequent, and patients with autoimmune hepatitis-2 exhibited higher disease remission rates with earlier response to treatment. Patients with autoimmune hepatitis-1 had a higher risk of death.     

Water polo is associated with an apparent redistribution of bone mass and density from the lower to the upper limbs

The bone response to exercise is site-specific and load-dependent. Recent evidence suggests that an inverse relationship may exist between loaded and unloaded sites, such that the former may benefit at the expense of the latter. The present study examined this possibility in 48 males (21 water polo players, 12 handball players, and 15 sedentary controls). Water polo and handball are alike with respect to the active loading of the upper limbs during overhead throwing; however, the weight-supporting environment of water polo removes the weight-bearing effect from the lower limbs. Bone mineral content (BMC), bone projected area (Ap), and areal bone mineral density (aBMD) of the total body and of various subregions were determined by dual-energy X-ray absorptiometry. After adjusting for age, height, and weight, water polo players had higher arms BMC, Ap, and aBMD (by 22.2, 11.1, and 10.5%, respectively; P < 0.05), but lower legs aBMD (−6.3%; P < 0.05) relative to controls. On the contrary, compared to controls, handball players had higher BMC (from 11.8 to 24.3%), Ap (from 5.2 to 11.7%), and aBMD (from 6.4 to 11.9%) for the total body at all sites. Water polo athletes had increased arms and decreased legs aBMD ratios (regional-to-total) than either handball players or sedentary subjects (P < 0.001). Water polo is associated with an apparent redistribution of bone mass and density from the lower to the upper limbs, with no major effects on the rest of the body.     

Does Distance Matter? Access to Family Planning Clinics and Adolescent Sexual Behaviors

The study examines the relationship between adolescent geographic access (distance, travel time, density) to Family Planning Clinics and adolescent sexual behaviors, including sexual initiation, number of partners and condom use. This cross-sectional study, conducted in 2005 in 10 California counties, utilized data from NICHD-funded study on adolescent sexual behavior (n = 921), geospatial coordinates of publicly-funded FPCs, and neighborhood characteristics. A series of regression models were used to assess the relationship between FPC distance, and density (number of FPCs within 1- and 3- mile radii of each adolescent’s home), and adolescent sexual behaviors. Significant main effects between access measures of FPC and sexual behavior did not emerge. However, among older youth a significant inverse relationship emerged between number of FPCs within a 1-mile radius and initiating sexual intercourse. While not significant at α = .10, the results also indicate a negative relationship between density of FPCs and sexual partners (b = −.22, p < .15) among older youth. Access to FPCs was not associated with condom use. Conclusions: Results suggest that increased options for family planning services may lead to less risky sexual behaviors among older youth. This finding has significant implications with regards to making family planning resources more readily available to older adolescents.     

Notes: Can We Use Imipenem and Meropenem Vitek 2 MICs for Detection of Suspected KPC and Other-Carbapenemase Producers among Species of Enterobacteriaceae?

Imipenem and meropenem Vitek 2 MICs were evaluated for a panel of 104 Enterobacteriaceae for identification of carbapenemase producers. The sensitivity and specificity values for the new CLSI interpretative criteria (CLSI document M100-S20-U, 2010) were 98% and 83% for imipenem and 76% and 83% for meropenem, respectively. We propose an algorithm that is highly sensitive (98%) and specific (94%) for carbapenemase screening based on the combined use of imipenem and meropenem MICs.